علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella ...
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Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella Orsini Jellyfish Venom According to Bloom method and was obtained its Concentration by Biuret method and Calculated LD50 by Jung and Choi method. According to Venom concentration and its LD50 was determined that Cause of death mice 0.5 ml of venom. The use of Na-EDTA for neutralizing venom. This Chelate Was injected In two ways to mice that In both methods, Prevented death. Na-EDTA is dedicated Chelate for Calcium excretion from body that According to nuclear calciums venom is able to separated that from Venom structure and neutralize venom.
علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate ...
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The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate the effect of temperature on the venom, it is heated at different temperatures and then injected into SoRI mice. After catching jellyfish Crambionella orsini from Arvand nidstream estuary umbrellas edge and tentacles jellyfish were separated and put were in water that was removed on the same area. Toxins LD50 were calculated by Jung and Choi method .and using statistical analysis to obtain minimal lethal dose of poison done by the 2007Excel. The results showed that the venome of jellyfish Crambionella orsini, like venome of other animals is, based on a protein and that is sensitive to heat. This venome at a temperature of 48 °C is disabled and lose their structure and also its minimum lethal dose is 0.5 ml.